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Image Search Results
Journal: PLoS ONE
Article Title: Studies on the non-invasive anticancer remedy of the triple combination of epigallocatechin gallate, pulsed electric field, and ultrasound
doi: 10.1371/journal.pone.0201920
Figure Lengend Snippet: The cell viability (24 h) was determined using MTT assay. (A) The HepG2 cells were triple treated with the EGCG (0 to 200 μM), 0.3 W/cm 2 US exposure (60 min), and consecutive 60 V/cm PEF for 24 h. The result revealed that the triple treatment could overcome the tolerance of cancer cells to EGCG and cause the death of HepG2 cells. (B) The triple treatment decreased the phosphorylation of Akt protein and increased the conversion of LC3-I to LC3-II in the HepG2 cells. The band intensities of LC3-II and LC3-I were quantified to calculate the LC3-II/LC3-I ratio. (C) The triple treatment triggered the activations of caspase-3 and PARP in the HepG2 cells. The cleaved PARP was quantified relative to the full-length PARP. β-actin was used as a loading control for each Western blot assay. (*** is used for P < 0.001).
Article Snippet: The human pancreatic cancer cell line PANC-1, the human
Techniques: MTT Assay, Phospho-proteomics, Control, Western Blot
Journal: World Journal of Gastroenterology
Article Title: Study on relationship between expression level and molecular conformations of gene drugs targeting to hepatoma cells in vitro
doi: 10.3748/wjg.v9.i9.1954
Figure Lengend Snippet: ELISA results of hIL-12 expressed in cell supernatant 48 hours after targeting gene drugs at various adjuvant concentrations were transfected into HepG2.
Article Snippet:
Techniques: Enzyme-linked Immunosorbent Assay, Adjuvant, Transfection